FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42079482
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT2576460.6122841531176643TruSeq Adapter, Index 14 (97% over 44bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA1168030.27757708614378857TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT302550.039.90744
AGATCGG174900.031.7155421
CGTATCT425500.027.93249939
TATCTCG428600.027.79718841
GATCGGA445450.027.785921
TTCCGTA446400.027.41809736
CCGTATC436150.027.34626438
GTTCCGT453850.026.94863735
AGTTCCG457200.026.82768234
ATCTCGT447450.026.59127242
ACACGTC468450.026.4876813
TCCGTAT457500.026.19511837
ATCGGAA477100.025.9909532
ACGTCTG477500.025.94419515
CGTCTGA478850.025.8261416
TCGGAAG485050.025.6686633
CACGTCT484550.025.65752414
CACACGT489300.025.38608712
GTATCTC472650.025.26705240
TCTCGTA476900.024.94459743
Produced by FastQC (version 0.11.5)