FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18201385
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC2494771.3706484424124867TruSeq Adapter, Index 3 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG619550.34038618489746797TruSeq Adapter, Index 3 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC281150.040.59497544
CATACGA28150.033.75964415
GCATACG29150.032.97883614
ATACGAG28900.032.73137316
TCGTATG349350.032.64484443
CGGCATA29600.032.40279412
CTCGTAT351050.032.35346642
GATCGGA363650.032.273711
ACACGTC368450.031.50656113
ATCTCGT357200.031.2060740
TCGGAAG376550.031.1470343
ACTTAGG368400.031.0990732
CACTTAG369700.030.94211231
CGTCTGA373750.030.90094416
ACGTCTG375300.030.86116215
TCTCGTA362150.030.82205641
CACGTCT377700.030.72330714
ATCGGAA382750.030.6470412
TAGGCAT365500.030.6359435
AGATCGG99750.030.6019971
Produced by FastQC (version 0.11.5)