FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21666361
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC508640.23476023500208454TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC71350.035.30408544
TCGTATG90450.027.92188643
CTCGTAT92550.027.19180542
GATCGGA98950.026.1275231
TCGAACG32800.024.95005840
TTGAACG35100.024.630827
ACACGTC107300.024.08943413
ATCGGAA112250.023.1516692
ATCTCGT107300.023.106140
ACGTCTG111200.023.1060915
TCGGAAG112950.022.9652483
CACGTCT115150.022.31348214
TCTCGTA112200.022.19504441
ACGGTAA37600.021.88251744
CACACGT119800.021.70436312
CGTCTGA119300.021.68484316
AGATCGG44250.021.6168561
GTCGAAC38700.021.37368239
CGAACGG39350.020.96471841
AACGGTA39450.020.85624343
Produced by FastQC (version 0.11.5)