FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19288574
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1592310.8255198129213699TruSeq Adapter, Index 4 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG595190.30857128163025427TruSeq Adapter, Index 4 (100% over 49bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG313580.16257293048205637Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA259270.13441636483858269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAG32550.034.26488516
CATACGA33500.033.88393415
CGGCATA35800.032.99726512
GCATACG34950.032.85581614
TATGCCG92400.031.3347973
TCGTATG261900.028.28250943
CTCGTAT263700.027.92942442
CGTATGC266850.027.80762944
TTGAACG42800.027.652277
GCCGTCT106350.027.3248396
GACCAAT271600.027.0534435
ATGCCGT107800.026.9572964
AATCTCG268850.026.84717639
ATCTCGT270650.026.79054840
ACCAATC274600.026.55739436
ACGAGAT41400.026.46191418
GTATGCC110500.026.2265262
TCTCGTA282550.025.71672441
GATCGGA300650.025.707051
TCGAACG45150.025.580240
Produced by FastQC (version 0.11.5)