FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32834403
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1104970.336528122652329TruSeq Adapter, Index 8 (100% over 50bp)
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA463750.1412390534403808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC135050.038.49437744
TCGTATG150350.034.66045443
CTCGTAT152450.034.009842
GATCGGA175150.030.5461041
ACACGTC178150.029.95958513
ATCTCGT173200.029.541940
AATCTCG174050.029.42290539
ACGTCTG184250.028.95576715
CACGTCT190600.028.04879614
TCTCGTA183400.028.00684441
ATCGGAA198600.027.0004122
TCGGAAG199100.026.9364913
TTGAACG83200.026.8633927
CGTCTGA201550.026.46668816
CACACGT203050.026.42653812
TCGAACG82350.026.28500640
GCACACG210700.025.63478511
TTGAATC210550.024.43713236
ACGGTAA89300.024.1682844
AACGCTG93150.023.83148410
Produced by FastQC (version 0.11.5)