FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36771437
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA765220.20810173940169918No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC517990.14086748907854757TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC71950.033.4314444
TTGAACG113800.029.7707217
TCGTATG87000.027.51055343
TCGAACG121200.027.40708440
AACGCTG124350.027.17343310
GAACGCT126450.026.704769
CTCGTAT90350.026.27131342
ACGGTAA127000.026.13143744
GTCGAAC131550.025.3343839
TGAACGC134550.025.19598
GAACGGT133600.025.04443642
GCCTAAC135500.024.5470824
AACGGTA136400.024.41749843
ATTGAAC146050.024.3266266
AGTCGAA137700.024.25081338
TCGACTA43950.024.0852944
CGAACGG139700.023.80914941
GATCGGA103150.023.3276941
GGCCTAA148500.022.5018723
GATTGAA166250.022.3760195
Produced by FastQC (version 0.11.5)