FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48585819
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT5412641.114036999149896TruSeq Adapter, Index 18 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA2529370.5205984075312181TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT603800.041.59245744
AGATCGG328850.035.6806031
GATCGGA900150.028.3937511
ACGTCCG895050.028.29328232
CGCACAT881300.028.07628337
AGTCACG901650.028.04300928
GTCACGT904850.027.91213829
GTCCGCA914100.027.74046534
ATCTCGT889150.027.68422742
TCACGTC913300.027.6803930
ACACGTC919700.027.67401113
TCTCGTA892050.027.63614743
ATCGGAA931750.027.4168992
TCCGCAC924600.027.41096535
ACGTCTG933300.027.26603115
TCGGAAG939600.027.2324473
CCGCACA935600.027.0863436
CGTCTGA941800.027.03090316
CACGTCT944650.026.95706214
CGTCCGC948050.026.760333
Produced by FastQC (version 0.11.5)