FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7307674
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAC6750.022.9122123
TACACTG40750.022.4804745
CGATACC20750.022.360802118-119
GCTACGA5000.021.4141082
GTCGTGT34900.021.3059028
GGGTCGT35450.021.1435816
GGTCGTG36650.020.9380867
TCGTGTG52250.020.6065189
CCTATAC20150.019.7786773
AGGGTCG39700.019.179265
CTATACT25800.018.9056514
GTATAGG15450.018.8661351
CGTATCG1908.341009E-418.784959
GTGTAAG36450.018.6046981
TATACTG39300.018.3149265
GTCTAGG34600.017.7082841
GTCTAGA46100.017.6780741
TAGACTG39050.017.5181875
GTCCTAT27300.017.4318181
GTATAGA19150.017.3954071
Produced by FastQC (version 0.11.5)