FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8194799
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG46350.019.6377535
GTACGGA6700.019.5356161
GTATAGG20550.017.949781
CGTACGG2050.001387527717.4131221
ACGGCGT6150.017.4121678
TATACTG46750.017.3064275
GTACTAG19350.017.2181431
CCTATAC23500.017.214583
GTGTAAG51200.017.0815241
GTATAGA23750.016.7838061
CTATACT29650.016.6537284
GTCCTAG35100.016.272091
TCGTGTG70750.016.1447099
GTCGTGT45100.015.5654218
TTACACT43450.015.472014
GTGTAGG38250.015.3986631
GGGTCGT46800.015.2538766
GGATCGT32850.015.2120846
CTAGACG9000.015.2034934
GGTCGTG46700.015.1591527
Produced by FastQC (version 0.11.5)