FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961739

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961739
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9110104
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCCG2055.948133E-520.3146825
GTATAGG22000.019.7429161
CTATACT31700.018.767924
TATACTG52200.018.6912865
TACACTG54750.018.3640465
CGATACG1151.4350038E-418.113075116-117
TCGTGTG79950.017.7848439
GTCCTAC25950.017.4255831
GTGTAGG40850.017.1870381
GGTCGTG57450.017.0865757
GGGTCGT56150.017.0583576
GTCGTGT56500.016.8477578
GTCCTAG38050.016.4189641
CCTATAC25700.016.2048513
CTAGTAC22300.016.007613
GTCCTAT34750.015.7523071
TCTATAC23800.015.7486633
GGATCGT31000.015.7366856
AGGGTCG61150.015.5664425
GTATAGC18100.015.4500681
Produced by FastQC (version 0.11.5)