FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9103017
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG4453.6379788E-1220.0492042
TATACTG53600.018.86465
CTATACT33900.018.7733944
GTATAGG24900.018.632481
GGGTCGT49550.018.3658586
TCGTGTG78500.018.2602239
GTACTAG21850.018.2389051
TACACTG54200.018.1070675
GTCCTAG40450.017.4986211
GGTCGTG50250.017.399827
TCTATAC23400.017.2842983
GTCGTGT49150.017.183978
TACACAG82650.016.9837135
GTATACG4251.7160346E-816.7945251
GGATCGT39350.016.4757526
TAGACTG50700.016.4241735
TATAGGG33400.016.3835222
AGGGTCG54500.016.1520885
GTGTAGG40850.016.016811
TCCGGAC12750.015.8608863
Produced by FastQC (version 0.11.5)