FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7548294
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT29550.023.1480454
CCTATAC21350.021.7305493
GTATAGG17600.021.6302411
TATACTG46200.021.6295265
GTGTAGG34900.020.9639821
GTACTAG17900.020.9354171
ATCGCGA1156.1479295E-620.69542140-41
GGCGTAC1754.795954E-420.394231
CCTACAC33050.019.9767783
TACACTG43250.019.8041445
TCTATAC24350.019.5418193
GGGTCGT51850.019.3870586
CGATACC17250.019.139458118-119
TCGTGTG76150.019.1364549
GGTCGTG51550.018.8075777
GTCGTGT51850.018.6985138
CCGTATA5101.8189894E-1218.6612632
ACGGCGT6150.018.3758188
GTCCTAG34950.017.7002851
AGGGTCG57850.017.479355
Produced by FastQC (version 0.11.5)