FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8194799
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAC5650.018.9489083
TCGTGTG68000.018.5430779
TAAGCGT8150.017.5151464
CGTAAAC7850.017.4268263
GTATAAG32550.017.1774311
GTATAGA24950.017.164981
GGGTCGT45350.016.9189176
GGTCGTG45900.016.8457687
ATCGTGT32800.016.6827968
GTGTAGG37250.016.6068941
CGCTATA4301.9828803E-816.5987592
GTCGTGT45150.016.5984548
GTACTAG18800.016.4523091
GTATATA61100.016.3549581
CTAGTAC20150.016.234833
CTATACT28100.016.0867164
GGATCGT32800.015.5949776
TACACTG45550.015.5392245
GTACGAC2300.002998950715.5161343
TATACAG39550.015.4903585
Produced by FastQC (version 0.11.5)