FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8412975
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG1751.7537166E-523.7977988
CCGTATA5500.023.7975142
GTATAGA24700.020.9560491
GTACGAC2909.695759E-820.5150973
GTGTAGG37750.020.015841
CTATACT30650.019.7990264
CCTATAC24350.019.5462133
CTAGTAC22000.018.1185633
TACACTG51400.018.056485
TATAGCG4059.433279E-917.6277892
TCTATAC24000.017.6002453
GTCCTAG37550.017.5873371
GTATAGG21700.017.5471781
GTACTAG20100.017.4639721
TAGTCCG2100.001630573116.9984269
TATACAG47250.016.7463995
GTATTAC24600.016.6878761
GTCGTAG4703.3160177E-916.4563141
TATACTG48450.016.454425
GTATATA68350.016.1905461
Produced by FastQC (version 0.11.5)