FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7741404
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG41700.023.3965975
GTACTAG19150.022.0583881
GGTCGTG38600.020.8061337
GGGTCGT40050.020.2013936
GTCGTGT39400.019.9309628
GTATACG3101.9574873E-719.19211
CGTATAC5700.018.7869643
TCGTGTG58900.018.5845939
GTATAGG20300.018.4641231
TATACTG39900.017.891775
GTGTAGG33800.017.7782441
AGGGTCG45950.017.6075255
GTACGAC2050.001387953617.4123083
TAGACTG42050.017.2599185
GTATTAC21950.017.076161
TACACAG69000.016.9848635
CCGTATA6051.8189894E-1216.7172072
CCTATAC19000.016.5951523
TATACGC3609.3943345E-716.5259972
CCTACAC29250.016.271333
Produced by FastQC (version 0.11.5)