FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7741404
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG41250.022.6398415
GGGTCGT39400.022.4945096
TATACTG40750.021.3119355
GGTCGTG40050.021.2383127
CTATACT28300.020.3877334
GTCGTGT40700.020.1678648
TCGTGTG59850.019.9759379
CCTATAC20400.019.8272463
GTATAGA22950.019.1807841
AGGGTCG45200.018.9505465
GTATACG3503.4247932E-818.6942623
GTGTAGG34650.018.54121
TCTATAC20300.018.459853
GTCTAGG35950.018.0361961
TTACACT38650.017.8522174
GTATAGG17000.017.8459051
TAGACTG38550.017.7448025
GTACTAG16250.017.2052841
GTCCTAG31850.016.809331
GTACCGT4251.715307E-816.7949526
Produced by FastQC (version 0.11.5)