FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7853839
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAC7500.022.21053
CCTACGA6150.020.3152
TACACCG4750.020.0390385
CTAGTAC18850.019.5677973
GGGTCGT44100.019.56056
TACACTG45350.019.2837095
GTACTAG18400.019.077831
GGTCGTG43850.018.9936737
TCGTGTG64550.018.8933129
GTCGTGT44150.018.460378
GTGTAGG36100.018.1292671
CTATACT29000.017.8477234
TATACTG41850.017.7690855
AGGGTCG49400.017.5823295
TAGACTG40650.017.4155395
CTACACT34300.016.9978334
TCTATAC20100.016.8709813
CGATACG1605.598751E-616.738209116-117
GCATACG4652.8776412E-916.6335581
CCTAGAC40500.016.5991173
Produced by FastQC (version 0.11.5)