FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8412975
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC2403.5789526E-722.3057353
CTATACT30250.020.4499954
TATACTG49100.019.9888465
TCGTGTG66450.018.4393089
GTCGTGT45100.018.3321428
GGGTCGT46450.018.1836136
GGTCGTG46600.018.1249737
TCTATAC23000.018.1032053
CGTAGCG3303.7844984E-718.0251582
GTCTAGG40600.018.0216831
CTACGAC7000.017.844593
CTTACAC44850.017.639023
AGGGTCG52300.016.4911755
GTGTAGG38450.016.3993171
GTATAGG20350.016.3696671
GTCCTAG37300.015.9480441
TAGACTG46100.015.8704585
TACACTG49250.015.8215995
TACGACA8850.015.4585894
CGATACG1354.887999E-415.424918116-117
Produced by FastQC (version 0.11.5)