FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4293314
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGACCTGACTAGTTTACAATCACACGACCCTGACCTGACTAGTTTA59710.13907671323364657No Hit
ACCCTGACCTGACTAGTTTACAACCACACGACCCTGACCTGACTAGTTTA53120.12372726523147387No Hit
GGTCAGGGTCGTGTGATTGTAAACTAGTCAGGTCAGGGTCGTGTGATTGT45330.10558277358702392No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACTA157850.020.796319
TCGTGTG148200.020.18429
ACCTGAC160700.020.0581267
GGGTCGT107000.019.9543656
GGTCGTG108550.019.8328767
GTCGTGT109000.019.6402728
GACCTGA167050.019.5813856
AGGGTCG111450.019.1576215
TGACCTG174200.019.016665
GGATCGT45200.018.8158936
TATAGCG2251.21898134E-418.5023782
ATCGTGT46350.018.474998
GATCGTG46750.018.0639927
GTACTAC8400.017.6997721
CCTGACT188050.017.3292088
TGTAACG3808.785173E-817.2155592
AGGATCG49200.017.1652625
TCTATAC10050.017.1610553
CTGACCT229550.017.04714
TACACTG24850.016.9925755
Produced by FastQC (version 0.11.5)