FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961894

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961894
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7681977
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC5250.021.5307663
TACACTG45850.020.5012385
GGCGTAC1600.00683748118.5931051
GTGTAGG35300.018.0347841
GTAGGAC21400.017.7922693
CTTACAC38250.017.7312183
CCTATAC20850.017.6909353
GTATAGG15900.017.587441
TCGTGTG52800.017.3519849
GTACTAG17250.017.2457791
TATACTG42550.017.1976015
CTACGAC6300.016.9979723
CTATACT27100.016.6843574
CGATACG1252.7142686E-416.663923116-117
GGTCGTG37250.016.450177
GGGTCGT37400.016.3841956
GTCGTGT36550.016.2770218
GTCTAGG34500.016.2110331
GTGTAAG39550.016.0967861
TATACAG36850.015.9830635
Produced by FastQC (version 0.11.5)