FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8457568
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC20950.020.4461293
CTATACT28800.019.830854
GTCCTAG32800.019.0472351
TATAGCG4458.0035534E-1118.7173122
GGGTCGT66650.018.47646
GGTCGTG66800.017.9005687
TACACTG47100.017.8093455
TCGTGTG102500.017.7022449
GTCGTGT66400.017.6502348
TATACTG46700.017.1975545
TCTATAC21150.017.158613
GTATAGG21000.016.999981
TAGACTG46050.016.9235485
GTGTAGG39450.016.7414261
AGGGTCG72900.016.4844175
CCGTATA4753.8180588E-916.2826542
TATACAG40200.016.1305875
GTATTAC24300.015.9156221
GTCCTAT32250.015.8666511
GTATAAG35100.015.7649411
Produced by FastQC (version 0.11.5)