FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8457568
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT30700.021.5048274
GTATAGG19300.020.6484221
CTAGTAC19350.019.979463
TCGTGTG91700.019.7175549
GGGTCGT59500.019.4926826
CCTATAC23850.019.4517023
GTCGTGT58650.019.369318
GGTCGTG60550.018.9580867
GTGTAGG43000.018.8122081
TACACTG48450.018.1685095
GTCCTAG38500.017.9211791
AGGGTCG66050.017.739645
GGATCGT40900.017.450666
CCGTATA4903.1650416E-1016.9935192
ATCGTGT41900.016.7500768
GTATAGA21750.016.6816841
GCTATAC16300.016.4201183
TCTATAC22200.016.3428673
TACACAG78950.016.2724515
CGTATAC7000.016.1438453
Produced by FastQC (version 0.11.5)