FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7853839
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG44950.022.6286985
GGGTCGT46250.021.7355636
CTATACT28950.021.162824
GTGTAGG36600.021.1286641
GTCGTGT45450.021.070338
TCGTGTG67800.020.8798129
GGTCGTG47050.020.7337287
AGGGTCG50600.019.749315
CCTATAC20300.019.6318343
TATACTG42650.019.3860325
CGATACC27200.018.808876118-119
GTACTAG17800.018.7144811
GTATAGG17850.018.328811
TATATCG2752.8754606E-517.3041575
GTGTTAG46750.016.541371
GTATAGA20500.016.539821
GTCGTAG4352.2835593E-816.4097421
GTATACG4352.2835593E-816.4097421
TACACAG68550.016.2265915
TACGACA6600.016.2223384
Produced by FastQC (version 0.11.5)