FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7548294
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT27350.023.7106484
GTGTAGG35100.022.8831231
TATACTG45250.021.5618045
TACACTG45650.021.3728735
CCGTATA5300.021.3283732
GGTCGTG49750.020.4482987
GGGTCGT48200.020.365316
GTCGTGT48200.019.9950318
GCTATAC15800.019.5803533
CGTATAC4900.019.426663
TCGTGTG74750.019.3396769
GTATAGG20400.019.2487431
AGGGTCG55150.018.6620985
CCTATAC19250.018.543633
TCTATAC20800.018.3058913
CCTACAC30700.018.2164483
CCTACGA5353.6379788E-1217.7928772
TACACAG72150.017.0684415
CTACACT36950.017.0673334
ACTGATC27000.016.9660648
Produced by FastQC (version 0.11.5)