FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6889099
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG2102.7968854E-622.6634888
TACACTG39100.022.5184175
GTACTAG15100.020.8834511
CTATACT23050.020.3899144
CCTATAC17300.019.9453243
GGGTCGT34100.019.0161196
CTAGTAC16450.018.806013
GGTCGTG33900.018.6018417
TCGTGTG50300.018.5689669
TATACAG34550.018.42425
TCTATAC17450.018.410163
GTCGTGT33800.018.3051248
TAGACTG35450.017.7886315
GTATAGA19950.017.595941
TACACAG60800.017.5146875
AGGGTCG38750.017.1948815
TATACTG38600.016.6452145
GTACGGA5851.4551915E-1116.2729951
GTCCTAG26700.016.267281
CTACACT32500.016.1086274
Produced by FastQC (version 0.11.5)