FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961938

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961938
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7307674
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACCG1107.7605963E-427.0422535
TACACTG40200.022.3468595
TATACTG38600.019.8823645
ATACCGT5150.019.6383196
TCGTGTG51700.019.5627529
GGGTACG5650.018.9553241
GGGTCGT34250.018.5859976
TATACAG34650.018.543265
CTATACT24900.018.3977994
GGTCGTG34050.018.1710017
GTCGTGT34400.018.1594378
TAGACTG36750.017.8074175
TCTATAC18950.017.5812743
TACCGTC3053.5555095E-617.5551827
AGGGTCG38750.017.0418555
GGATCGT26000.016.9324976
GTGTAGG29300.016.854571
CTACGAC5657.2759576E-1216.8477953
GCTATAC14150.016.8180273
ACTGATC27400.016.7190558
Produced by FastQC (version 0.11.5)