FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4962377
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCG850.007216840527.996662
TACACTG31800.022.2630675
CGCTATA2204.186375E-621.6337832
CGTACAC3451.2878445E-920.6929763
CCGTACA2909.6952135E-820.5147932
ACCTGAC106550.020.1566757
CTGACTA105750.020.1407939
TATACTG29750.019.9976145
GACCTGA111000.019.9381626
CGTCGTA2550.019.83636338-39
CTATACT18750.019.672324
CGTATAG3953.274181E-1019.5797443
CGTATCG1550.00569403419.1916478
TGACCTG116600.019.1336525
GCTATAC9950.019.1332023
TATACAG26450.018.4439245
CCCGTAC2601.772005E-518.3062461
TCTATAC14950.018.3053253
ACTAGCG4258.6947693E-1018.197832
CCGTATA2952.586141E-618.1503772
Produced by FastQC (version 0.11.5)