FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4962377
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG2558.8584784E-1025.661641
TCGTGTG97350.021.080679
GTCGTGT71900.020.6004228
GGTCGTG71400.020.5784727
GTGTAAG26200.020.4349291
TGTAGCG4102.5465852E-1120.3114572
GGGTCGT70950.020.2897826
TATACGC2408.900895E-619.827852
AGGGTCG75150.019.4724465
GTACTAG12300.019.3458041
CTATACT21850.019.3289474
TACACTG30000.019.2337915
GTATAGG11550.019.0568731
TAGACTG27000.018.9473765
GGATCGT33850.018.8035356
TCGTAGG2551.5016778E-518.6615052
GTGTAGG21250.018.4763811
ATCGTGT33300.018.3991878
TCTATAC13950.018.3356173
CCTATAC14650.018.2715823
Produced by FastQC (version 0.11.5)