FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004961958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004961958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10168955
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCGT70350.020.0386566
TATACTG58950.019.6759265
GGTCGTG70750.019.2527797
GTCGTGT71800.018.8880128
TACACTG61150.018.8707715
GTACTAG24900.018.6337831
TCGTGTG108900.018.570649
GTCCTAG46400.018.332591
AGGGTCG76950.018.1653445
CTATACT36350.017.836024
TATGCGT7050.017.7176484
GTGTAAG57050.017.412721
GTATTAC31800.017.3964881
GTATAGG23450.016.9956471
GTGTAGG48050.016.8364811
CTACGAC7300.016.2960723
GTACGAC3751.441791E-615.8615123
GTATATA73050.015.6345991
TCTATAC28750.015.5166963
GTCCTAC29850.015.3444791
Produced by FastQC (version 0.11.5)