FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004962021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8622544
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG47000.019.4898935
TCGTGTG62300.017.3769
GTGTAGG37250.017.247331
GGGTCGT38750.017.1922596
TCTATAC24450.017.0295523
GTCCTAG39100.016.8877051
GTCGTAG4251.713488E-816.796421
TATACTG46350.016.5549015
GGATCGT32350.016.5483556
CTATACT30150.016.3747584
GGTCGTG40350.016.2157027
GTCGTGT38800.015.9431128
GTATAGG20200.015.9025511
GTGCTAC23250.015.8632861
GTCCTAT32950.015.5262811
CTAGGAC37500.015.385963
TATACAG41950.015.313645
GTGTAAG48200.015.1803751
GTACTAG18200.015.0352571
ATCGTGT32350.014.8930028
Produced by FastQC (version 0.11.5)