FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004962022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7543515
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC20200.019.7339611
GTACTAG18150.019.6682381
CTATACT26500.019.0824954
GTATTAC20850.018.2626911
CCGTACA4651.4915713E-1017.912192
GTATAAG28850.017.7355021
TAGACGA6450.017.524455
TCGTGTG68750.017.133729
GTCGTGT44900.017.092388
CTATACG4903.1468517E-1016.9978524
TACACTG42100.016.9570275
GGGTCGT45900.016.8492766
TCTATAC20550.016.7910653
GGTCGTG46000.016.683327
TATACAG38050.016.4166745
GTATAGA20450.016.292421
GTGTAAG41300.016.2786831
TAGACTG39700.016.1839245
TATACTG45300.016.1531585
GTGTAGG33400.016.0319711
Produced by FastQC (version 0.11.5)