FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004962025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4008494
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCA1653.2301815E-421.6234829
CCTATAC11500.021.2014713
TCGTGTG63150.019.9627699
GGATCGT27850.019.858356
GTACGAC1500.00472278819.8225153
GGGTCGT38550.019.282846
GATCGTG29750.018.9893997
GGTCGTG39550.018.7948177
GTCGTGT39450.018.5398228
GTCTTAG18400.018.421321
ATCGTGT28450.018.39288
TACACTG25100.018.243265
CTATACT14100.018.1352654
CGTATAG2652.0958281E-517.9524673
TACGACC3003.04871E-617.840044
AGGGTCG41650.017.7048455
GTCCTAG16050.017.0430181
CTGACTA61100.016.9342659
AGGATCG32150.016.8323925
CTAGGAC16000.016.7252463
Produced by FastQC (version 0.11.5)