FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004962031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9746794
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC28650.021.3891283
GTGTAGG44100.020.6433491
CGTATCG2158.579071E-519.3695728
TATACTG55750.018.887935
GTATAGG20950.018.461061
GTACTAG20550.018.2413081
CTATACT35300.018.0338044
TACACTG54300.017.4202065
TACGACT9850.016.9121994
CTACGAC7400.016.8837173
CGTACAC8300.016.4865683
TCGTGTG87350.016.2777889
CCTACGA8150.016.0603312
GGGTCGT58650.015.92536
GTCTAGA58450.015.8806171
GGTCGTG58450.015.8780117
GTATTAC29250.015.6636211
CCTAGAC47100.015.6632283
GTCCTAG42300.015.473181
GCGTATA3851.8913688E-615.4535642
Produced by FastQC (version 0.11.5)