FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004962047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8867342
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT31300.024.3247874
GTCGTAG4605.456968E-1219.3967551
TCGTGTG72100.019.0562789
GTCGTGT47350.018.9679688
GGTCGTG47800.018.9143647
GGGTCGT48250.018.4915146
CGTTACG2902.2018485E-618.4589029
TCTATAC24250.018.151123
TATACTG50600.017.6329175
AGGGTCG52050.017.3702585
CGATACG1201.9913145E-417.353775116-117
CCTATAC22350.017.0327933
TACACTG48650.016.9947725
TCGTAGG4251.7154889E-816.794932
GTCTAGG42650.016.7362381
GTATACG4652.8867362E-916.6297631
GTATCCG3951.3634599E-716.5649931
GTATTAC27000.016.523161
GTCTAGA52650.016.4949171
GTAACGC15300.016.3274829
Produced by FastQC (version 0.11.5)