FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004962067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9110104
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG22200.019.2915251
TCGTGTG71250.018.7813789
CTATACT28950.018.4899444
GGTCGTG52600.018.0916567
TATACTG48000.017.9669045
GGGTCGT53550.017.881776
GTGTAGG39450.017.641111
GTCGTGT52700.017.6054128
AGGGTCG57750.016.8903395
TACACTG51400.016.778435
TATACAG45350.016.5249355
CTACGAC6850.016.4969923
GCTATAC16650.016.4318073
GTCTAGG40550.016.4291151
GTCCTAG38200.016.349821
GGATCGT30100.016.202856
GTATTAC24550.015.991141
GTAACGC14550.015.9415819
GGCGTAC2250.002589134715.8619191
CGATATG3451.1506589E-515.5150519
Produced by FastQC (version 0.11.5)