FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004962127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7685601
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG44150.022.6379535
GCTATAC14350.021.9732253
GTATAGG18400.020.3711
GTGTAGG32350.018.759251
CTAGTAC19200.018.2818723
TACACAG68800.018.1588945
CTTACAC39500.018.0739883
GGGTCGT32950.018.0552356
TCGTGTG50950.017.865239
TAGACTG37450.017.7925
GTCGTGT34100.017.620918
GGTCGTG35650.017.3553097
TATACAG37650.017.2234465
CCTACAC28000.017.2106343
CCGTACA4151.2754754E-817.202844
TATACTG44000.017.1715555
AGTACCG7000.016.9977135
CTATACT28450.016.93834
CTACACT36400.016.671164
CGATACG1252.715194E-416.66318116-117
Produced by FastQC (version 0.11.5)