FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004962128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7685601
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGA1852.699461E-522.5065946
GGGTCGT29000.021.94676
TACACTG40900.021.5235255
GTCGTGT28350.021.4004128
TCGTGTG43900.020.7299889
GGTCGTG30000.020.6203277
TATACTG42550.020.5490975
GTCCTAG32200.019.7660481
CTACGAC6750.019.3858743
CTATACT27950.019.3653814
GTATAGG18500.019.2917421
AGGGTCG33750.019.0337755
TAGACGA5650.017.8968035
CGTATCG2000.001177738717.8441648
GTACTAG18600.017.5890221
CCTACGA5800.017.4342962
GTGTAAG40100.017.2070161
CGATACC20100.017.165497118-119
CCTATAC20000.016.654233
CTACACT35050.016.4607734
Produced by FastQC (version 0.11.5)