FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004962144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6889099
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG38550.022.682115
GGTCGTG38300.022.6750227
GGGTCGT38450.022.4318586
GTCGTGT38500.021.7844078
CTACGAC5900.021.1713373
TCGTGTG59350.020.946579
AGGGTCG42350.020.5064245
TATACTG37050.020.0683785
CGATACC24050.019.292797118-119
CTATACT24200.018.4343134
GTACTAG15850.018.38991
TAGGCCG6800.018.369655
GTATAGG17150.018.0364781
CCTACGA5002.1827873E-1117.845192
TACCGTC2351.6984086E-417.718377
CGTATCG1700.00971181317.4947768
CTAGTAC17250.017.2409783
GGGTACG5204.0017767E-1117.1593361
TAGACTG37550.017.1081985
ACTGATC22600.017.1077238
Produced by FastQC (version 0.11.5)