FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004962223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20496847
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGA6650.014.9976499
CGTTTCG9800.012.1155364
AGACGGG170150.011.4795181
AGGACCG25700.011.4649551
TCGAACG8851.8189894E-1211.269428
AGGGCCG57050.010.912631
AGGGACG50450.010.7388431
AGGGACA257350.010.6368281
ACCCGTT21950.010.6090231
AGCGACG12550.010.6029831
CGGGGTT145950.010.5756724
ACGGGGT147150.010.3281783
AGGGGCG66200.010.193991
GACGGGG162750.010.0678162
AGGGGTT154400.010.0650111
AGGGTTT271750.010.0382061
AGAGACG149250.010.0302091
AGGGTCG27100.09.9958421
AGGGAAT194200.09.9599911
AGGGGAC159050.09.890271
Produced by FastQC (version 0.11.5)