FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004962229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20913722
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA38700.020.3896451
TCGGAAG55450.013.8755412
TCTCGTA33450.013.06636742-43
TCGTATG32850.012.94231244-45
CGGAAGA59550.012.3618753
CTCGTAT30350.012.052999542-43
TATGCCG28600.011.875339546-47
CAATCCG37850.011.86390732-33
CGTATGC31800.011.20361944-45
ATGCCGT40600.010.76364848-49
ATCCGGA42700.010.51616734-35
ACACGTC45900.09.62275612-13
CCGGAAT48050.09.44403936-37
ATCTCGT42350.08.69457340-41
AATCCGG44700.08.344954532-33
GCCGTCT56650.08.00736750-51
TGCCGTC49350.07.84455348-49
AATCTCG62250.07.746877740-41
GTATGCC59600.07.49182546-47
CGGAATC51450.07.342256536-37
Produced by FastQC (version 0.11.5)