FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004962285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24254680
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTATCGCACATCTCGTAT670430.2764126345925817TruSeq Adapter, Index 9 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA98950.068.129831
ATCGGAA100900.066.934562
TCGGAAG128150.052.7383423
CGGAAGA149050.045.375164
TCGTATG85750.039.16563444-45
TATGCCG86450.038.4913848-49
CTCGTAT86600.038.36981644-45
ACTATCG87900.038.2337332-33
CGTATGC87650.037.99110846-47
TATCGCA91000.037.03585434-35
TCTCGTA91700.036.77983542-43
CTATCGC94950.035.62001832-33
ATGCCGT99800.033.69946748-49
CGCACAT104150.032.6563636-37
ATCTCGT103100.032.45959542-43
TCGCACA104550.032.16794236-37
ACACGTC110450.030.7877912-13
ATCGCAC115100.029.59069634-35
AGAGCAC232250.029.4059568
GTATGCC115350.029.19741846-47
Produced by FastQC (version 0.11.5)