FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004962301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21902184
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAATATATCTCGTAT433980.19814462338550348TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA67550.065.137641
ATCGGAA71350.062.171262
TCGGAAG92550.048.237773
CGGAAGA104550.042.6557274
TATGCCG61000.035.6662348-49
CTCGTAT61600.035.5116244-45
TCGTATG64150.034.35918444-45
TCTCGTA64500.034.0989442-43
CGTATGC64300.034.02034846-47
TATCTCG69500.031.54332540-41
ATGCCGT71400.031.03666348-49
CGTGAAT73300.030.23133732-33
ATCTCGT74800.029.43524742-43
ACACGTC77950.028.48363112-13
CGTCTGA82700.026.73286616-17
AGTCACG85850.025.89422228-29
GTCACGT86150.025.80404728-29
TGCCGTC87100.025.11487850-51
AGAGCAC183850.024.5408638
GCCGTCT93150.023.81516650-51
Produced by FastQC (version 0.11.5)