FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004962340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21119008
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT276640.13099100109247555TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA51650.050.844531
ATCGGAA52400.050.127932
CGTACGT36500.035.9177734-35
CCGTACG38750.034.13850832-33
TCGTATG39050.033.3273744-45
GTACGTA40750.032.69628534-35
CTCGTAT40050.032.61382344-45
TCGGAAG86200.030.9681283
ACCGTAC43800.030.09400232-33
TATGCCG43200.030.01587148-49
ACGTAAT44450.029.65354536-37
TCTCGTA44600.029.18023542-43
CGTATGC44450.029.11838746-47
TACGTAA44950.029.11235236-37
CGGAAGA99600.027.0401844
CGTAATC52200.025.20495638-39
CACCGTA55950.024.32285730-31
ATGCCGT55700.023.79145848-49
ATCTCGT56500.023.1604142-43
ACACGTC64150.020.73145512-13
Produced by FastQC (version 0.11.5)