FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004962342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23072721
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA21450.029.3382361
ATCGGAA28350.022.7865582
TCGGAAG37600.017.4334813
CGTTTCG18650.017.19138132-33
TATGCCG20200.016.22437748-49
CGGAAGA43400.014.7753214
CTCGTAT21700.014.33672644-45
CGTATGC23850.013.44270446-47
TCTCGTA23650.012.75297442-43
TCGTATG26150.012.078649544-45
TCGGAAT27000.011.6107436-37
GTTTCGG27900.011.57689134-35
ATGCCGT30350.010.9549348-49
TGCCGTC30600.010.86538250-51
TTCGGAA30800.010.64089336-37
ACACGTC33150.09.95785812-13
GCCGTCT31850.09.84244350-51
TTTCGGA32800.09.63019434-35
ATCTCGT34300.09.34713642-43
GTCACGT35150.09.32379328-29
Produced by FastQC (version 0.11.5)