FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004962386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21427084
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGACG38600.013.7883361
AGGGCCG34600.012.0861431
AGGGGCG38050.011.8645181
AGACCCG22450.011.8536691
GCCTTCG71450.011.8313233
CGTTTCG10850.011.8180974
AGAGACG116150.011.4556551
AGGGAAT262500.011.441131
AGGGTCG19200.011.3851231
AGGCGCC63850.011.089361
AGGGGTT138950.011.080711
AGACGCG8601.4551915E-1111.0512821
AGTCCCG22800.011.0464351
AGGACCG20650.011.0459321
AGGGGTC109250.010.9177571
CCTTCGT77950.010.9056224
AGACGGG127550.010.8788631
AGGGGAC117750.010.815711
CGCCACC83700.010.7804438
AGGGTAC69800.010.7567931
Produced by FastQC (version 0.11.5)