FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004962387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20000881
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGCG149100.013.6406631
AGGGCCG101600.013.5167881
CGTTTCG21900.013.2283734
AGGGCGG145500.012.0512681
ACCCGTT43850.011.703711
AGTCCCG61450.011.6768161
AGGGGCC210600.011.3044321
AGGGTCG43800.011.2831051
AGGGCGC100900.011.0674111
AGGACCG42000.010.9746791
AGGGACG76700.010.6562021
AGGGAAT267850.010.6445911
AGCGCCG65750.010.4072531
AGACGGG153750.010.2919691
AGGAAAT339400.010.2766981
AGGGTAC63150.010.2337531
ATCTTCG64400.010.1030668
AGACGAA33400.010.1013931
AGAGACG134050.010.0674821
AGCGTCG19400.010.0427231
Produced by FastQC (version 0.11.5)