FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004962388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20803109
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGCG42150.012.8523691
AGGGTCG18950.011.785951
AGGGACG42150.011.7249691
ATCTTCG55050.011.55973158
AGGGAAT262450.011.3707471
AGGGCCG38250.011.0569281
AGGGTCT213300.011.0501141
AGGCGCC70400.010.6649881
AGTCGAT11150.010.65469551
AGTCCCA322700.010.5583511
AGGGACA215900.010.542871
AGACGGG133350.010.5124741
AGGGGTT134000.010.4260181
GCCTTCG73100.010.3946583
AGGGACT152000.010.316831
AGGGGCC137050.010.2980111
CGCCACC96450.010.2906868
AGGGTCC109300.010.2604831
AGGGGAC118500.010.2258111
AGGGGTC109100.010.1921791
Produced by FastQC (version 0.11.5)