FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004962405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20960360
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCCG155250.014.664221
AGGGGCG242750.014.175371
AGGGACG104350.013.5275481
AGGGCGG220650.012.4718171
AGTCCCG93500.011.8948591
AGAGACG148700.011.3787381
AGGGAAT234950.011.1058541
AGGGCGC158450.011.0685111
AGGGGCC273450.011.0543771
AGGACCG62350.010.9769351
AGGGTCG70500.010.9214671
AGGGAGC266250.010.7463731
AGGAGCG133100.010.7126831
AGGGACC187900.010.6996281
AGGGACT166100.010.6731911
AGGGGAG399650.010.4535581
AGGGGGC296500.010.4514841
AGACGTC32450.010.3991721
AGGCGCC189250.010.2717051
CGTTTCG24100.010.2472574
Produced by FastQC (version 0.11.5)