FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004962488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21080521
Sequences flagged as poor quality0
Sequence length57
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTC865910.41076309261995947TruSeq Adapter, Index 8 (100% over 57bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTT732230.34734910014795173TruSeq Adapter, Index 8 (100% over 56bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG109300.035.725731
CGTCTTC154350.029.67148451
TCGAACG30900.027.2328440
TTGAACG35100.025.572317
TCGTATG178650.025.37839743
CGTATGC180950.025.08400244
TATGCCG180800.025.04845646
CTCGTAT182150.024.89075342
CGAACGG34750.024.2156241
GATCGGA195350.023.6053871
ATCTCGT196550.023.09321640
AACGGTA36700.022.99838643
ACGGTAA37050.022.98760444
ATGCCGT200000.022.89880447
AATCTCG199200.022.82435239
ACACGTC202350.022.82178713
GTATGCC199600.022.81693845
GTCGAAC37150.022.6512239
ATCGGAA205900.022.3916852
GCCGTCT204300.022.27970549
Produced by FastQC (version 0.11.5)