FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487124948
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG14182000.2911368029543007TruSeq Adapter, Index 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2754750.086.628561
TCGGAAG2951300.080.856932
CGGAAGA3169550.075.2275163
AGAGCAC5968750.040.3120467
AGCACAC6277000.038.3784989
GAGCACA6443000.037.4504438
GAAGAGC6544950.036.904825
AAGAGCA9247350.026.4397076
GGAAGAG10445750.023.4848884
TATGCCG2114500.022.82594545-49
CTCGTAT2092900.021.52192740-44
CGTATGC2261100.021.17544245-49
AGCGATA2281650.021.11256830-34
TCTCGTA2141100.020.996840-44
CGATAGA2271900.020.94809735-39
GCGATAG2284850.020.85198830-34
ATGCCGT2416600.019.97726645-49
TCGTATG2263450.019.73307240-44
TGCCGTC2439500.019.59063345-49
GCCGTCT2482300.019.22903350-54
Produced by FastQC (version 0.11.5)